Composite
Part:BBa_K733018:Design
Designed by: WANG, Yuqi Group: iGEM12_HKUST-Hong_Kong (2012-09-16)
xylR+PxylA+RBS+GFP+Double Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 847
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2058
Design Notes
None.
Source
We digest and ligate our xylR+PxylA and BBa_E0240 from 2012 Kit Plate.
Backbone: xylR+PxylA in pSB1C3. Enzymes used: SpeI and PstI-HF.
Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation.